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pmcorrect

PM Correction


Description

Corrects the PM intensities in a ProbeSet for non-specific binding.

Usage

pmcorrect.pmonly(object)

pmcorrect.subtractmm(object)

pmcorrect.mas(object, contrast.tau=0.03, scale.tau=10, delta=2^(-20))

Arguments

object

An object of class ProbeSet.

contrast.tau

a number denoting the contrast tau parameter in the MAS 5.0 pm correction algorithm.

scale.tau

a number denoting the scale tau parameter in the MAS 5.0 pm correction algorithm.

delta

a number denoting the delta parameter in the MAS 5.0 pm correction algorithm.

Details

These are the pm correction methods perfromed by Affymetrix MAS 4.0 (subtractmm) and MAS 5.0 (mas). See the Affymetrix Manual for details. pmonly does what you think: does not change the PM values.

Value

A ProbeSet for which the pm slot contains the corrected PM values.

References

Affymetrix MAS 4.0 and 5.0 manual

Examples

if (require(affydata)) {
  data(Dilution)
  gn <- geneNames(Dilution)
  pps <- probeset(Dilution, gn[1])[[1]]

  pps.pmonly <- pmcorrect.pmonly(pps)
  pps.subtractmm <- pmcorrect.subtractmm(pps)
  pps.mas5 <- pmcorrect.mas(pps)
}

affy

Methods for Affymetrix Oligonucleotide Arrays

v1.68.0
LGPL (>= 2.0)
Authors
Rafael A. Irizarry <rafa@ds.harvard.edu>, Laurent Gautier <lgautier@gmail.com>, Benjamin Milo Bolstad <bmb@bmbolstad.com>, and Crispin Miller <cmiller@picr.man.ac.uk> with contributions from Magnus Astrand <Magnus.Astrand@astrazeneca.com>, Leslie M. Cope <cope@mts.jhu.edu>, Robert Gentleman, Jeff Gentry, Conrad Halling <challing@agilixcorp.com>, Wolfgang Huber, James MacDonald <jmacdon@u.washington.edu>, Benjamin I. P. Rubinstein, Christopher Workman <workman@cbs.dtu.dk>, John Zhang
Initial release

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