Download FASTA Sequence Files
Downloads FASTA sequence files from the NCBI nr, SWISSPROT/UNIPROT, OR RCSB PDB databases.
get.seq(ids, outfile = "seqs.fasta", db = "nr", verbose = FALSE)
ids |
A character vector of one or more appropriate database codes/identifiers of the files to be downloaded. |
outfile |
A single element character vector specifying the name of the local file to which sequences will be written. |
db |
A single element character vector specifying the database from which sequences are to be obtained. |
verbose |
logical, if TRUE URL details of the download process are printed. |
This is a basic function to automate sequence file download from the databases including NCBI nr, SWISSPROT/UNIPROT, and RCSB PDB.
If all files are successfully downloaded a list object with two components is returned:
ali |
an alignment character matrix with a row per sequence and a column per equivalent aminoacid/nucleotide. |
ids |
sequence names as identifiers. |
This is similar to that returned by read.fasta
. However,
if some files were not successfully downloaded then a vector detailing
which ids were not found is returned.
For a description of FASTA format see: https://www.ncbi.nlm.nih.gov/BLAST/blastcgihelp.shtml. When reading alignment files, the dash ‘-’ is interpreted as the gap character.
Barry Grant
Grant, B.J. et al. (2006) Bioinformatics 22, 2695–2696.
## Not run: ## Sequence identifiers (GI or PDB codes e.g. from blast.pdb etc.) get.seq( c("P01112", "Q61411", "P20171") ) #aa <-get.seq( c("4q21", "5p21") ) #aa$id #aa$ali ## End(Not run)
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