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intra-range-methods

Intra range transformations of a RangedSummarizedExperiment object


Description

This man page documents the intra range transformations that are supported on RangedSummarizedExperiment objects.

Usage

## S4 method for signature 'RangedSummarizedExperiment'
shift(x, shift=0L, use.names=TRUE)

## S4 method for signature 'RangedSummarizedExperiment'
narrow(x, start=NA, end=NA, width=NA, use.names=TRUE)

## S4 method for signature 'RangedSummarizedExperiment'
resize(x, width, fix="start", use.names=TRUE,
       ignore.strand=FALSE)

## S4 method for signature 'RangedSummarizedExperiment'
flank(x, width, start=TRUE, both=FALSE,
      use.names=TRUE, ignore.strand=FALSE)

## S4 method for signature 'RangedSummarizedExperiment'
promoters(x, upstream=2000, downstream=200)

## S4 method for signature 'RangedSummarizedExperiment'
restrict(x, start=NA, end=NA, keep.all.ranges=FALSE,
         use.names=TRUE)

## S4 method for signature 'RangedSummarizedExperiment'
trim(x, use.names=TRUE)

Arguments

x

A RangedSummarizedExperiment object.

shift, use.names

See ?shift in the IRanges package.

start, end, width, fix

See ?shift in the IRanges package.

ignore.strand, both

See ?shift in the IRanges package.

upstream, downstream

See ?shift in the IRanges package.

keep.all.ranges

See ?shift in the IRanges package.

Details

These transformations operate on the rowRanges component of the RangedSummarizedExperiment object, which can be a GenomicRanges or GRangesList object.

More precisely, any of the above functions performs the following transformation on RangedSummarizedExperiment object x:

rowRanges(x) <- f(rowRanges(x), ...)

where f is the name of the function and ... any additional arguments passed to it.

See ?shift in the IRanges package for the details of how these transformations operate on a GenomicRanges or GRangesList object.

See Also

Examples

nrows <- 20; ncols <- 6
counts <- matrix(runif(nrows * ncols, 1, 1e4), nrows)
rowRanges <- GRanges(rep(c("chr1", "chr2"), c(5, 15)),
                     IRanges(sample(1000L, 20), width=100),
                     strand=Rle(c("+", "-"), c(12, 8)))
colData <- DataFrame(Treatment=rep(c("ChIP", "Input"), 3),
                     row.names=LETTERS[1:6])
rse0 <- SummarizedExperiment(assays=SimpleList(counts=counts),
                             rowRanges=rowRanges, colData=colData)

rse1 <- shift(rse0, 1)
stopifnot(identical(
  rowRanges(rse1),
  shift(rowRanges(rse0), 1)
))

se2 <- narrow(rse0, start=10, end=-15)
stopifnot(identical(
  rowRanges(se2),
  narrow(rowRanges(rse0), start=10, end=-15)
))

se3 <- resize(rse0, width=75)
stopifnot(identical(
  rowRanges(se3),
  resize(rowRanges(rse0), width=75)
))

se4 <- flank(rse0, width=20)
stopifnot(identical(
  rowRanges(se4),
  flank(rowRanges(rse0), width=20)
))

se5 <- restrict(rse0, start=200, end=700, keep.all.ranges=TRUE)
stopifnot(identical(
  rowRanges(se5),
  restrict(rowRanges(rse0), start=200, end=700, keep.all.ranges=TRUE)
))

SummarizedExperiment

SummarizedExperiment container

v1.20.0
Artistic-2.0
Authors
Martin Morgan, Valerie Obenchain, Jim Hester, Hervé Pagès
Initial release

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