Peptide to protein mapping.
This function performs peptide to protein mapping given a peptide expression matrix and a peptide to protein map.
pep2prot(pep.Expr.Data, rollup.map)
pep.Expr.Data |
Peptide expression data matrix. |
rollup.map |
Peptide to protein map. |
A protein expression matrix. Each line is obtained by aggregating the lines in the peptide expression matrix which have the same value in the rollup.map.
Cosmin Lazar
# generate expression data matrix
dataObj = generate.ExpressionData(nSamples1 = 6, nSamples2 = 6,
meanSamples = 0, sdSamples = 0.2,
nFeatures = 2000, nFeaturesUp = 100, nFeaturesDown = 100,
meanDynRange = 20, sdDynRange = 1,
meanDiffAbund = 1, sdDiffAbund = 0.2)
exprsData = dataObj[[1]]
# insert 15% missing data with 100% missing not at random
m.THR = quantile(exprsData, probs = 0.15)
sd.THR = 0.1
MNAR.rate = 100
exprsData.MD.obj = insertMVs(exprsData,m.THR,sd.THR,MNAR.rate)
exprsData.MD = exprsData.MD.obj[[2]]
# generate rollup.map
rollUpMap = generate.RollUpMap(round(dim(exprsData.MD)[1]/2),exprsData.MD)
# peptide to protein mapping
protExprsData = pep2prot(exprsData.MD,rollUpMap)
## The function is currently defined as
function (pep.Expr.Data, rollup.map)
{
protIDs = unique(rollup.map)
prot.Expr.Data = matrix(, nrow = length(protIDs), ncol = dim(pep.Expr.Data)[2])
for (i in 1:length(protIDs)) {
temp = protIDs[i]
pepSet = pep.Expr.Data[which(rollup.map == temp), ]
if (length(which(rollup.map == temp)) == 1) {
prot.Expr.Data[i, ] = pepSet
}
else {
prot.Expr.Data[i, ] = apply(pepSet, 2, median, na.rm = T)
}
}
return(prot.Expr.Data)
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