Peptide to protein mapping.
This function performs peptide to protein mapping given a peptide expression matrix and a peptide to protein map.
pep2prot(pep.Expr.Data, rollup.map)
pep.Expr.Data |
Peptide expression data matrix. |
rollup.map |
Peptide to protein map. |
A protein expression matrix. Each line is obtained by aggregating the lines in the peptide expression matrix which have the same value in the rollup.map.
Cosmin Lazar
# generate expression data matrix dataObj = generate.ExpressionData(nSamples1 = 6, nSamples2 = 6, meanSamples = 0, sdSamples = 0.2, nFeatures = 2000, nFeaturesUp = 100, nFeaturesDown = 100, meanDynRange = 20, sdDynRange = 1, meanDiffAbund = 1, sdDiffAbund = 0.2) exprsData = dataObj[[1]] # insert 15% missing data with 100% missing not at random m.THR = quantile(exprsData, probs = 0.15) sd.THR = 0.1 MNAR.rate = 100 exprsData.MD.obj = insertMVs(exprsData,m.THR,sd.THR,MNAR.rate) exprsData.MD = exprsData.MD.obj[[2]] # generate rollup.map rollUpMap = generate.RollUpMap(round(dim(exprsData.MD)[1]/2),exprsData.MD) # peptide to protein mapping protExprsData = pep2prot(exprsData.MD,rollUpMap) ## The function is currently defined as function (pep.Expr.Data, rollup.map) { protIDs = unique(rollup.map) prot.Expr.Data = matrix(, nrow = length(protIDs), ncol = dim(pep.Expr.Data)[2]) for (i in 1:length(protIDs)) { temp = protIDs[i] pepSet = pep.Expr.Data[which(rollup.map == temp), ] if (length(which(rollup.map == temp)) == 1) { prot.Expr.Data[i, ] = pepSet } else { prot.Expr.Data[i, ] = apply(pepSet, 2, median, na.rm = T) } } return(prot.Expr.Data) }
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